Efficacy of DNA Extraction Methods Using Simple Boiling and Commercial Kit in Extracting Streptococcus Mutans in Caries
Keywords:
DNA Extraction, Dental Caries, Genetics, Streptococcus mutansAbstract
The rapid progression of molecular-based technology now enables us to analyse huge number of samples. Nonetheless, DNA (Deoxyrebonucleic acid) extraction is always a limiting factor. In this study, we analysed human microbes to compare the performance of DNA extraction methods: simple boiling method and MasterPureTM Complete DNA and RNA Purification Kit. Dental plaque was initially collected from 12 subjects, 6 of which were from individuals with caries active lesions with International Caries Detection and Assessment System (ICDAS) score five and six, and 6 samples from non-caries subjects, were collected in deionised water. The bacterial samples were extracted by the two aforementioned methods and examined using gel electrophoresis, followed by polymerase chain reaction (PCR). Streptococcus mutans was detected using conventional polymerase chain reaction (PCR). Our results demonstrated that boiling method produced higher DNA concentration (100 – 350 ng/μl), however, the commercial kit yield superior in DNA quality with single and specific PCR products. Based on the findings, the commercial kit is the better choice and practicable method for DNA extraction considering the quality of the DNA yield.
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