Exploring Genetic Traits and variation in Pattani Malay population using T-ARMS PCR, HRM and Sanger Sequencing
Keywords:
T-ARMS PCR · Pattani Malay · HRM analysis · Sanger sequencing · Genotype frequencyAbstract
This study investigates the genetic polymorphisms of the Pattani Malay population, emphasizing the distribution of genetic variations and their potential implications for their health and susceptibility to region-specific diseases. The study utilizes T-ARMS PCR, High-Resolution Melt (HRM) analysis, and Sanger sequencing to explore genetic traits and variations within the population. The HRM method effectively genotyped samples for the SNP rs961378, revealing three distinct variants, predominantly exhibiting a heterozygote genotype with genotype frequency of 0.60. Sanger sequencing verified the genetic traits of variants classification from HRM analysis, which include heterozygous, homozygous T and homozygous C. The study adhered to Hardy Weinberg Equilibrium with chi-squared value 0.40, providing insights into the distribution of genotypes. However, the limitation of a small sample size with 10 samples requires caution in interpretation, as it may lead to false positives and lack of representativeness. Validation in a larger sample size is recommended to ensure the reliability and generalizability of the findings. The study identifies challenges associated with the T-ARMS PCR method, emphasizing the need for improvements in primer design and contamination prevention. Despite offering valuable insights, the study highlights the importance of cautious interpretation due to the limitations. It suggests further research involving a larger, more diverse sample size and methodological enhancements to establish a better understanding of genetic associations in the Pattani Malay population, contributing to broader knowledge in the field.
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