Molecular Characterization of Hypervariable Region I and II in Mitochondrial DNA of Nusantara Malays
Keywords:
Mitochondrial DNA, Nusantara Malay, sequence polymorphism, genetic diversity, mtDNA haplogroupAbstract
Previously, population and genetic studies on the Nusantara Malays are not widely known and discovered despite their dispersal pattern. The Malay-Archipelago, also known as Nusantara, is a part of the Austronesian (Malayo-Polynesian) linguistic family. The aim of this study is to identify the mtDNA profile for the Nusantara Malays. Blood samples were collected from maternally unrelated individuals (n=26) from five sub-ethnic groups of Nusantara Malays residing in Peninsular Malaysia were analysed to establish the sequence polymorphism and genetic diversity. MtDNA parameters were estimated, and the observed polymorphisms with their respective haplogroups in comparison to rCRS were inferred, respectively. The mtDNA sequences of HVI and HVII in 26 unrelated Nusantara Malay individuals were evaluated. Molecular diversity indices and differentiation tests were computed. HVI haplotypes were unique for each Nusantara Malay sample while three HVII haplotypes were shared. All Nusantara Malay individuals were grouped in haplogroup H. This data will enhance the DNA database of the Malay population, which can be used for elucidating the history of Nusantara Malays expansion in Peninsular Malaysia.
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